National University of Ireland, Maynooth

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Faculties & Departments

Department of Biology

Professor Sean Doyle         
Head of Laboratory

Biotechnology Laboratory

Professor Sean DoyleResearch Interests

Our molecular and applied proteomics work, which has resulted in over 50 peer-reviewed publications, is directed towards understanding microbial pathogenicity and identifying new biotechnological, therapeutic and diagnostic targets. Our group also has a number of academic and industrial collaborations and group members have been pro-active in the promotion of the public understanding of science through participation in such activities as the NUI Maynooth Chain Reaction Lecture Series and the National Science Week. Research work in the laboratory is funded by the Higher Education Authority (HEA-PRTLI), Enterprise Ireland, Science Foundation Ireland, Teagasc and The Irish Research Council for Science, Engineering and Technology (IRCSET).

Research area 1

Aspergillus fumigatus: We study the biology, diagnosis and molecular genetics of the human pathogenic fungus, Aspergillus fumigatus - a major cause of infection in immunocompromised individuals. Our group has identified a number of potential virulence factors (proteins) in the organism, which may contribute both to its pathogenicity and emerging resistance to conventional chemotherapies. We were the first to identify and characterise a novel self-protection mechanism against gliotoxin in Aspergillus fumigatus. We have also identified a number of non-ribosomal peptide synthetases and glutathione s-transferases in A. fumigatus. Interestingly, non-ribosomal peptide synthetases in fungi represent a novel source of bioactive metabolites and our published work is at the cutting-edge of this internationally competitive area. The primary research methods involved in this work comprise gene deletion studies, proteomics, NMR and LC-MS.

Collaborators: Dr Kevin Kavanagh, Dr Gary Jones and Dr John Stephens (NUI Maynooth), Dr Markus Schrettl and Professor Hubertus Haas (Innsbruck, Austria), Dr Elaine Bignell (Imperial College, London), Professor Thomas Larsen (Copenhagen, Denmark).

Research area 2

Armillaria mellea: In the past, this plant pathogenic fungus has proven recalcitrant to detailed molecular analysis. However, we have undertaken genome sequencing of this organism and have established a robust proteomics pipeline to identify novel biocatalysts (enzymes) with biotechnological, pharmaceutical and biofuel applications. This work involves conventional individual protein mass spectrometry and shotgun proteomics. The primary research methods involved in this work comprise genome sequencing, bioinformatics, proteomics, HPLC and small molecule LC-MS.

Collaborators: Dr David Fitzpatrick (NUI Maynooth), Dr Thomas Keane and Dr Dan Turner (UK).

Research area 3

Immunodiagnostics: Using a well-established recombinant protein expression systems (E. coli and baculovirus) we have expressed a number of A. fumigatus proteins in order to assess their diagnostic potential. In addition, we have developed novel immunoassays to detect fungal metabolites as disease indicators. Proteomic studies of a number of organisms, using Departmental protein mass spectrometry facilities, are also well underway and peer-reviewed publications are now emerging from these efforts.

Research equipment infrastructure available for Industry-Academia Collaborations

The Department of Biology possesses a range of modern molecular and proteomic instrumentation which is used for both PhD and Post-Doctoral Research projects. This equipment, much of which has been funded by competitive awards from the HEA, HRB, SFI and Enterprise Ireland, is also available for use by companies on a commercial basis. For further details see:

http://biology.nuim.ie/documents/index.shtml

Laboratory Personnel

Postdoctorate

Postgraduate

PhD Student Awards

A number of PhD students supervised directly, or collaboratively, by Professor Doyle have won national and international awards for the quality of their research and presentation skills. Further details of these awards can be found at the links below:

1. Research Award for NUI Maynooth PhD Student in Chemical Biology.

http://graduatestudies.nuim.ie/news-events/features/chemistry

2. Biology PhD student wins Research Excellence Award.

http://graduatestudies.nuim.ie/node/402

3. Joint Teagasc/NUI Maynooth PhD Student Wins Research Award.

http://graduatestudies.nuim.ie/newsevents/jointteagascnuimaynoothphdstudentwinsresearchaward

4. NUI Maynooth PhD Biology student wins prestigious award.

http://graduatestudies.nuim.ie/newsevents/nuimaynoothphdbiologystudentwinsprestigiousaward

5. NUI Maynooth Biology student wins international award.

http://communications.nuim.ie/university_news/documents/june-newsletter-final_000.pdf

6. Competitive National University of Ireland Travelling Studentship Awarded to Biology PhD Student - November 2010

http://biology.nuim.ie/documents/NUITravellingFellowshipAward-060111.pdf

7. NUI Maynooth Biology PhD Student Wins Award for Infectious Disease Research - June 2011

http://biology.nuim.ie/documents/BiologyPhDStudentAward-RO-160611.pdf

 

Previous MSc & PhD Graduates

PhD & MSc Graduates (2000 to date) Current Position     

  • Dr Amanda Corcoran* - Senior R&D Scientist, Biotrin.
  • Dr Sinead Waters - Senior Scientist, Teagasc.
  • Dr Claire Neville* - Senior Technical Specialist, Olympus.
  • Dr Elaine McCarthy - Senior Scientist, Ildana Biotech.
  • Janet Clynes MSc - Secondary School Teacher.
  • Dr Imelda Vickers - Post-Doctoral Research Fellow, Temple St, Hospital.
  • Rachel Geraghty MSc - Senior Data Analyst, Quintiles.
  • Dr Deirdre Stack* - Post-Doctoral Research Fellow, St James Hospital.
  • Carol Davis MSc - PhD Student, NUI Maynooth.
  • Dr Stephen Carberry - Post-Doctoral Research Fellow, NUI Maynooth.
  • Dr Sheena Fagan - Senior Scientist, Alltech.
  • Dr Luke O’Shaughnessy - Post-Doctoral Research Fellow, NUI Maynooth.
  • Dr Mark Gaffney - Senior Scientist, Alltech.
  • Olivia Johnston MSc.
  • Valerie Fitzgerald MSc - Technical Officer, Dublin City University.
  • Dr. Eilish Lynch - Research Officer, Irish Cancer Society.
  • Dr. Laura Anne Byrne -Senior Scientist, Alltech.
  • Dr. Lorna Gallagher - Post-Doctoral Research Fellow, USA.
  • Dr. Karen O'Hanlon - Post-Doctoral Research Fellow, Denmark.
  • Dr. Carol Davis - Post-Doctoral Research Fellow, USA.
  • Dr. Grainne O'Keeffe
  • Dr. Sarah Keady
  • Dr. Aran O'Loughlin

Past Post-Doctoral Research Fellows/Staff

  • Dr Orla Ennis
  • Dr Paul Daly
  • Dr Colin Stack
  • Dr Emer Reeves
  • Dr Kathrin Reiber
  • Dr Claire Burns
  • Dr Tony O’Connor
  • Dr Markus Schrettl
  • Dr Christopher Jochl
  • Dr. Stephen Carberry
  • Dr. Emer Molloy
  • Kieran Walshe, M.A. (Mod.)
  • Dr. Natasha Gordon
  • Dr. Luke O'Shaughnessy

Current Funding Sources

HEA PRTLI-4, NUI, IRCSET, Teagasc and Departmental funding.

Previous funding

HEA PRTLI 3, European Union, Biotrin Limited, Tridelta Development Limited, Biosensia Limited, Enterprise Ireland Awards (Innovation Partnerships, Strategic Research, Proof of Concept), HRB, Canopus Biopharma and Enterprise Ireland (Proof of Concept), Health Research Board, Science Foundation Ireland, Enterprise Ireland (Technology Development Awards).

Patent Applications

  • Walshe K, Doyle S, Gordon N, Kavanagh K, and Gallagher L (2011) Method for detecting infections. Irish Patent application. Filed 16 May 2011.
  • Davis C, Kavanagh K and Doyle S. (2009) Detection and Diagnostic Methods. Irish Patent Application P920781IE00. Filed 15 January 2009.
  • Schrettl M., Carberry S, Kavanagh K. Haas H and Doyle S. (2008) Selection marker for transformation of fungi. Filed 22 September 2008.
  • Doyle JM and Stack D. 24 January 2008. "Method for detecting phosphopantetheinyl transferase activity". European Patent Application No. 08100863.3.
  • McCarthy C and Doyle JM. Diagnostic Method. S2007/0801. Submitted November 2007.
  • Walshe K and Doyle JM 2001. Non-immunological assays for the detection and determination of C-Reactive Protein. PCT/IE 01/00124. Date of filing: 27th Sept 2001.
    (Funding: Irish-American Partnership MSc Studentship. ‘C-Reactive Protein detection by turbidimetry’ co-funded by Enterprise Ireland / Tridelta Development Limited and Enterprise Ireland Innovation Partnership Grant. ‘C-Reactive Protein Development of a novel detection product and manufacturing process’ -co-funded by Tridelta Development Limited.)

Selected Publications

  • O'Loughlin A., Lynn D.J., McGee M., Doyle S., McCabe M. and Earley B. (2012) Transcriptomic analysis of the stress response to weaning at housing in bovine leukocytes using RNA-seq technology. BMC Genomics. In press.
  • O’Hanlon K.A., Margison G.P., Hatch A., Fitzpatrick D.A., Owens R., Doyle, S., Jones G.W.  (2012). Molecular characterization of an adaptive response to alkylating agents in the opportunistic pathogen Aspergillus fumigatus. NAR. In press.
  • Carberry, S., Molloy, E., Hammel, S., O’Keeffe, G., Jones, G.W., Kavanagh, K. and Doyle, S. 2012. Gliotoxin Effects on Fungal Growth: Mechanisms and Exploitation. Fungal Genet Biol. 49: 302-312.
  • O’Hanlon K.A., Gallagher, L., Schrettl, M., Jöchl C., Kavanagh K., Larsen T.O. and Doyle S. (2012). Non-Ribosomal Peptide Synthetases pesL and pes1 are essential for Fumigaclavine C production in Aspergillus fumigatus. Applied Environ Microbiol.  79(8): 3166-3176.
  • Marques MPC, Walshe K., Doyle S., Fernandes P. and de Carvalho CCCR, (2012) Anchoring high-throughput screening methods to scale-up bioproduction of siderophores Process Biochemistry. Process Biochemistry. 47(3): 416–421.
  • O’Shaughnessy, L., Carr, M., Crowley, B., Carberry, S. and Doyle S. (2011) Recombinant Expression and Immunological Characterisation of Proteins Derived from Human Metapneumovirus. J. Clin Virol. 52: 236-243.
  • Davis C., Gordon, N., Murphy S., Singh I., Kavanagh K, Carberry, S. and Doyle S. (2011) Single-Pot Derivatisation Strategy for Enhanced Gliotoxin Detection by HPLC and MALDI-ToF Mass Spectrometry. Anal. Bioanal Chem. 401: 2519-29.
  • O’Hanlon K.A., Cairns T., Stack D., Schrettl M., Bignell E.M., Kavanagh K., Miggin S.M., O’Keeffe, G., Larsen, T.O. and Doyle S. (2011) Targeted Disruption of Non-Ribosomal Peptide Synthetase pes3 Augments the Virulence of Aspergillus fumigatus. Infect. Immun. 79: 3978-3992.
  • O'Loughlin, A., McGee, M., Waters, S.M., Doyle, S. and Earley B. (2011) Examination of the bovine leukocyte environment using immunogenetic biomarkers to assess immunocompetence following exposure to weaning stress BMC Vet Res, 7:45 doi:10.1186/1746-6148-7-45.
  • Doyle, S. (2011) Fungal proteomics: from identification to function. FEMS Microbiol. Lett.  321(1):1-9.
  • Davis, C., Carberry, S., Schrettl, M., Singh, I., Stephens, J.C., Barry, S.M., Kavanagh, K., Challis, G.L., Brougham, D., Doyle, S. (2011) The Role of Glutathione S-transferase GliG in Gliotoxin Biosynthesis in Aspergillus fumigatus. Chem. Biol. 18:542-552.
  • Lynch EM, Earley B, McGee M, Byrne W and Doyle S. (2010) Characterisation of physiological, haematological, and immunological responses in beef cows to abrupt weaning and subsequent housing. BMC Veterinary Research. 6:37.
  • Lynch EM, Earley B, McGee M, Byrne W and Doyle S. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves. BMC Veterinary Research. 6:39.
  • Schrettl, M, Carberry, S, Kavanagh, K, Haas, H, Jones GW, O’Brien J, Stephens J, Fenelon O, Nolan A and Doyle, S. (2010) Self-Protection against Gliotoxin- A Component of the Gliotoxin Biosynthetic Cluster, GliT, Completely Protects Aspergillus fumigatus Against Exogenous Gliotoxin. PLOS Pathogens. 6(6): e1000952. doi:10.1371/journal.ppat.1000952.
    Available as Immediate Open Access on  http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1000952
  • Gaffney, M., Doyle, S. and Murphy R. (2009) Optimisation of xylanase production by Thermomyces lanuginosus in solid state fermentation. Bioscience, Biotechnology and Biochemistry. 73, 2640-2644.
  • Gaffney, M., Carberry, S., Doyle S. and Murphy, R. (2009). Purification and characterisation of a xylanase from Thermomyces lanuginosus and subsequent expression by Pichia pastoris. Enzyme and Microbial Technology, 45, 348-354.
  • Stack, D., Frizzell, A., Tomkins, K. and Doyle, S. (2009) Solid Phase 4’-Phosphopantetheinylation- Fungal Thiolation Domains are Targets for Chemoenzymatic Modification. Bioconj. Chem., 20, 1514-1522.
  • Corcoran A., Kerr S., Koppelman M. and S. Doyle (2007). Improved Detection of Acute Parvovirus B19 Infection by IgM EIA in Combination with a Novel Antigen EIA. Vox Sanguinis. 93, 216-222.
  • Carberry, S. and Doyle S. (2007) Proteomic studies in biomedically and industrially relevant fungi. Cytotechnology. 53(1-3), 95-100.
  • Stack D., Neville C.M. and Doyle S (2007). Nonribosomal Peptide Synthesis in the Human Pathogen Aspergillus fumigatus and other Fungi. Microbiology. 153: 1297-1306. Available as Immediate Open Access on http://mic.sgmjournals.org/content/vol153/issue5/
  • Vickers I., Reeves E.P., Kavanagh K.A. and Doyle S. (2007) Isolation, activity and immunological characterisation of a secreted aspartic protease, CtsD, from Aspergillus fumigatus. Protein Expr Purif. 53(1): 216-224.
  • Reeves EP, Reiber K, Neville C, Scheibner O, Kavanagh K and Doyle S. (2006) A nonribosomal peptide synthetase (pes1) confers protection against oxidative stress in Aspergillus fumigatus. FEBS J. 273(13): 3038-3053.
  • Doyle S. and Corcoran A. (2006) The Immune Response to Parvovirus B19 Exposure in Previously Seronegative and Seropositive Individuals. J Infect Dis. 194(2):154-8.
  • Carberry, S., Neville, C.M., Kavanagh, K.A. and Doyle S. (2006) Analysis of major intracellular proteins of Aspergillus fumigatus by MALDI mass spectrometry: Identification and characterisation of an elongation factor 1B protein with glutathione transferase activity. Biochem Biophys Res Commun. 341(4):1096-104.
  • Corcoran, A., Crowley, B., Dewhurst, C., Pizer, BL., and Doyle S. (2006) Establishment of Functional B Cell Memory against Parvovirus B19 Capsid Proteins may be associated with Resolution of Persistent Infection. J. Med. Virol. 78(1):125-8.
  • Reiber, K., Reeves, E., Neville, C., Winkler, R., Gebhardt, P., Kavanagh, K. and Doyle, S. (2005) The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron. FEMS Microbiol. Lett. 248(1):83-91.
  • Waters, S.M., Doyle, S. , Murphy, R.A. and Power, R.F.G. (2005) Development of solution phase hybridisation PCR-ELISA for the quantification of Enterococcus faecalis and Pediococcus pentosaceus in Nurmi-type cultures. J Microbiol Methods. 63(3):264-75.
  • Corcoran, A. and Doyle, S. (2005) Evidence of serological cross-reactivity between genotype 1 and genotype 3 erythrovirus infections. J Virol. 79(8). 5238-5239.
  • Burns, C., Geraghty, R., Neville, C., Murphy A., Kavanagh K. and Doyle S. (2005) Identification, cloning and functional expression of three glutathione transferase genes from Aspergillus fumigatus. Fungal Genet Biol. 42(4):319-327.
  • Neville, C., Murphy, A. Kavanagh, K. and Doyle S. (2005) A 4'-phosphopantetheinyl transferase mediates non-ribosomal peptide synthetase activation in Aspergillus fumigatus. ChemBiochem. Feb 18; 6(4):679-685.
  • Wallace K, Elrick L, Leel V, Durward E, Doyle S, Kavanagh K, Porter A, Wright MC. (2005) Targeting therapies for the treatment of liver fibrosis - Chemical conjugation of gliotoxin to a human small chain antibody selected for its affinity to a surface antigen on human hepatic stellate cells. Hepatology 42 (4), 601A-602A Suppl. 1.
  • Corcoran, A. and Doyle, S. (2004) Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19. J Med Microbiol. 53(Pt 6): 459-475.
  • Corcoran, A., Mahon, B.P. and Doyle, S. (2004) B cell memory is directed towards conformational epitopes of parvovirus B19 capsid proteins and the VP1-unique region. J Infect Dis. 189(10):1873-1880.
  • McCarthy E., Stack, C., Donnelly, S.M., Doyle, S., Mann, V.H., Brindley, P.J., Brennan, G., Stewart, M., Maule, A.G., and Dalton, J.P. (2004) Leucine aminopeptidase of the human blood fluke, Schistosoma mansoni. Int J Parasitol. 34(6): 703-714.
  • Collins P.R., Stack C.N., O'Neill S.M., Doyle S., Ryan T., Brennan G.P., Mousley A, Stewart M, Maule AG, Dalton JP, Donnelly S. (2004) Cathepsin L1, the major protease involved in liver fluke (Fasciola hepatica) virulence: Propeptide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells. J Biol Chem. 279(17): 17038-17046.
  • Reeves, E.P., Messina, G.C.M., Doyle, S. and Kavanagh, K. (2004) Correlation between Gliotoxin Production and Virulence of Aspergillus fumigatus in Galleria mellonella. Mycopathologia. 158(1):73-79.
  • Fox, M., Gray, G., Kavanagh, K., Lewis, C. and Doyle, S. (2004). Detection of Aspergillus fumigatus mycotoxins: immunogen synthesis and immunoassay development. J Microbiol. Methods. 56(2): 221-230
  • O'Keeffe, J., Doyle, S. and Kavanagh, K. (2003). Exposure of the yeast Candida albicans to the anti-neoplastic agent Adriamycin increases the tolerance to Amphotericin B. Journal of Pharmacy and Pharmacology. 55 (12):1629-33.
  • Dalton, J.P., O'Neill, S.M., Stack, C., Collins, P., Walsh, A., Sekiya, M., Doyle, S., Mulcahy, G., Hoyle, D., Khaznadji, E., Moiré, N., Brennan, G.P., Mousley, A., Kreshchenko, N., Maule, A.G. and Donnelly, S.M. (2003) Fasciola hepatica cathepsin L-like proteases: biology, function, and potential in the development of first generation liver fluke vaccines. International Journal for Parasitology. 33(11);1173-1181.
  • Corcoran A., Mahon B.P., McParland P., Davoren A. and Doyle S. (2003) Ex vivo cytokine responses against parvovirus B19 antigens in previously infected pregnant women. J. Med Virol. 70(3):475-480
  • Daly P and Doyle S (2003) The development of a competitive PCR-ELISA for the detection of Equine Herpesvirus-1. Journal of Virological Methods. 107: 237-244.
  • Deegan O, Walshe K, Kavanagh K, and Doyle S. (2003) Quantitative Detection of C-Reactive Protein Using Phosphocholine - Labelled Enzyme or Microspheres. Anal. Biochem. 312(2): 175-181.
  • Daly P., Corcoran A., Mahon B.P. and Doyle S. (2002). High Sensitivity PCR Detection of Parvovirus B19 in plasma. J. Clin. Microbiol. 40(6): 1958-1662.
  • Raab U, Beckenlehner K, Lowin T, Niller H-H, Doyle S and Modrow S (2002) NS1-protein of Parvovirus B19 interacts directly and via the transcription factors Sp1/Sp3 with the p6 promoter. Virology 293(1):86-93.
  • Daly P, Collier T and Doyle S (2002) PCR-ELISA detection of Escherichia coli in milk. Letters in Applied Microbiology. 34(3):222-6.
  • O. Ennis, A. Corcoran, K. Kavanagh, B.P. Mahon and S. Doyle (2001) Baculovirus expression of parvovirus B19 (B19V) NS1: Utility in confirming recent infection. Journal of Clinical Virology 22:55-60.
  • Walshe K and Doyle JM (2001). Non-immunological assays for the detection and determination of C-Reactive Protein. PCT/IE 01/00124. Date of filing: 27/9/01.
  • Corcoran A, Doyle S, Waldron D, Nicholson A, and Mahon BP. (2000) Impaired Interferon- g Responses against Parvovirus B19 By Recently Infected Children. Journal of Virology 74, 9903-9910.
  • Cotter G, Doyle S and Kavanagh K (2000) Development of an insect model for the in vivo pathogenicity testing of yeasts. FEMS Immunology and Medical Microbiology. 1165: 1-7.
  • Doyle S, Kerr S, O'Keeffe G, O'Carroll D, Daly P and Kilty C (2000) Detection of parvovirus B19 IgM by antibody capture enzyme immunoassay: Receiver Operating Characteristic analysis. Journal of Virological Methods. 90(2), 143-152.
  • Kerr S, O'Keeffe G, Kilty C and Doyle S (1999) Undenatured Parvovirus B19 antigens are necessary for the accurate serodiagnosis of Parvovirus B19 IgG. Journal of Medical Virology. 52: 179-185.
  • Eckersall PD., Duthie S, Safi S, Moffatt D, Horadagoda NU,
    Doyle S, Parton R, Bennett D and Fitzpatrick JL (1999) ‘An automated biochemical assay for haptoglobin: prevention of interference from albumin.' Comparative Haematology International 9: (3) 117-124.
  • Flendrig L., Chamuleau RAFM., Maas MAW, Daalhuisen J, Hassett B, Kilty CG, Doyle S, Ladiges NCJJ, Jorning GGA, la Soe JW, Sommeijer D and te Velde AA (1999) Evaluation of a novel design bioartificial liver in rats with complete liver ischemia: treatment efficacy and species-specific ?-GST detection as a first attempt to monitor hepatocyte viability. Journal of Hepatology. 30(2), 311-321.
  • Kilty C, Doyle S, Hassett B and Manning F (1998) Glutathione s-transferases as biomarkers of organ damage: applications of rodent and canine GST enzyme immunoassays. Chemico-Biological Interactions 112, p123-135.
  • Clarke, H, Egan, DA., Heffernan, M, Doyle, S, Byrne, C, Kilty, C and Ryan, MP (1997) Alpha-glutathione s-transferase (alpha-GST) release, an early indicator of carbon tetrachloride hepatotoxicity in the rat. Human Experimental Toxicology, 16 (3), 154-157.
  • Hobson, AH, McCormack, C, Doyle, S, Jackson, J, Kilty, C and Whitehead, A (1996) Generation of soluble recombinant human acute phase serum amyloid A2 protein and its use in the development of an A-SAA specific ELISA. J. Immunol. Methods. 198, 101-110.
  • Rees, GW, Trull, AK and Doyle, S (1995) Evaluation of an enzyme-immunometric assay for serum glutathione s-transferase. Annals Clin. Biochem. 32, 575-583.
  • Doyle JM, Schinina ME, Bossa F, Doonan S (1990) The amino-acid-sequence of cytosolic aspartate-aminotransferase from human liver Biochem J. 270: (3) 651-657.

Book Chapters

  • O'Shaughnessy L and Doyle S. (2011) Purification of proteins from baculovirus-infected insect cells. Methods Mol Biol. 681:295-309. Invited review.
  • Doyle S. (2011) The Detection of Parvoviruses in “Diagnostic Virology Protocols”. Humana Press. Editors: Dr. John Stephenson and Dr. Alan Warnes. Methods Molecular Biology. 665: 213-31. Invited review.
  • Doyle S. (2010) ‘Fungal Proteomics’ in Fungi: Biology and Applications. Wiley. Editor. K. Kavanagh. Submitted 2 December 2010. Invited review.
  • Doyle S. (2009) Nonribosomal peptide synthesis in “Amino acids, Peptides and Proteins in Organic chemistry. Vol 2 –modified amino Acids, Organocatalysis and Enzymes”. 978-3-527-32098-1. Wiley-VCH. Published 23 September 2009. Editor: Andrew Hughes. Invited review.
  • Corcoran A. and Doyle S. (2006) Human Parvovirus B19: Molecular Virology, Clinical Features, Prevalence, Diagnosis and Control in “Congenital and Other Related Infectious Diseases of the Newborn” (Perspectives in Medical Virology), ISBN: 0-444-52073-2.  Academic Press (Elsevier BV). Editor I. K. Mushahwar. p 173-209.
  • C.G. Kilty and S. Doyle (1996) Alpha ‘Glutathione s-transferases as biomarkers of organ viability and damage’ in Glutathione s-Transferases: Structure, Function and Clinical Implications. Taylor & Francis Limited, London. Eds: NPE Vermeulen, GJ Mulder, Nieuwenhuyse WHM Peters and PJ van Bladeren. p 249-258. ISBN: 0-7484-0331-0.

Patents Granted

  • Doyle JM, Hobson HA and Whitehead AS. Method for the quantitative measurement of human acute phase serum amyloid a protein; recombinant protein; specific antibody. EP00842434B1. Issued 15/3/2000.
  • Kilty, CK, Doyle JM and Manning F. Method of determining the hepatic status of an individual, including a liver transplant recipient. EP00880700A1. Issued: 2/12/1998.
  • Kilty CK and Doyle JM. Method of determining or detecting donor organ damage following xenotransplantation based on donor organ-derived analytes. EP00871881A1.  Issued 21/10/1998.
    Kilty CK and Doyle JM. Rapid assays for the assessment of organ status based on the detection of one or more isoenzymes of glutathione s-transferase. WO09631779A1 Issued: 10/10/1996.
  • Kilty C.K and Doyle JM. Stabilising medium for alpha-glutathione s-transferase in urine for use in an enzyme immunoassay. WO09612191A1 Issued 25/04/1996.
Last edited: Monday, 14-May-2012 13:34:17 IST

Biology Department , NUI Maynooth
Tel: +353-1-708 3843 | Fax: +353-1-708 3845| Email: terry.roche@nuim.ie